Human Genetic Variation by National Institute of Health - HTML preview

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Introduction

variation. The primary vehicle is a class inventory of human traits that

highlights similarities and differences. Although variation, both phenotypic

and genotypic, is the central focus of all five lessons, this concept is less

explicit in subsequent lessons than it is in this one.

One goal of the Human Genome Project was to provide the complete

sequence of the human genome. Another goal was to illuminate the extent

of human genetic variation by providing a detailed picture of human

similarities and differences at the molecular level. Research indicates that

any two individuals are 99.9 percent identical at the level of their DNA.

The 0.1 percent where we vary from one another (about 1 out of 1,000

DNA bases) is clearly very important. It is within this small fraction of

the genome that we find clues to the molecular basis for the phenotypic

differences that distinguish each one of us from all others.

In this lesson, students are introduced to the notion that although we are

very similar to one another, we are also very different and our differences

reflect a complex interplay between genetic and environmental factors.

This understanding sets the stage for subsequent lessons in the module in

which students learn about the molecular differences that help explain our

phenotypic differences and also consider some of the medical and ethical

implications of scientists’ growing understanding of these differences.

Web-Based Activities

In Advance

None.

Materials and Preparation

Photocopies and Transparencies

Equipment and Materials

• 1 copy of Master 1.1 for

• plant, fish, prepared slide of

each student

bacteria

• 1 copy of Master 1.2 for

• masking tape for labeled axes

each student

on the board or wall in which

students can enter data

• 120 3 × 5 cards (4 per student;

required only if the axes are on

the wall)

• tape measure (1 per pair of

students)

Construct four sets of axes on the board or the classroom wall (use masking

tape). Label the axes as shown in Figure 7.

64

Figure 7. Construct the four sets of axes shown here on the board or on a wall of your classroom.

1. Begin the lesson by telling the class something like, “If a visitor from

Procedure

another planet walked into this classroom, he might easily conclude

that humans all look very much alike.” If students complain that this

is not true, answer with something like, “You certainly are more like

one another than you are like this plant [point to the plant]. Or this

fish [point to the fish]. And for sure, you are more alike than any one

of you is like the bacteria on this slide [wave the prepared slide of

bacteria in the air]. Humans— Homo sapiens—have a set of traits that

define us as a species, just like all other species have a set of traits

that define them.”

2. Continue the lesson by saying, “Let’s see just how similar you are.”

Give one copy of Master 1.1, An Inventory of a Few Human Traits,

to each student and ask students to work in pairs to complete it.

If students are unfamiliar with the following terms, provide the

definitions below.

detached earlobes: Earlobes hang free, forming a distinct lobe.

hitchhiker’s thumb: Most distal joint of thumb can form almost a

90-degree angle with the next most proximal joint.

middigital hair: Hair is present on digits distal to knuckles.

cross left thumb over right: Natural tendency is to cross left thumb

over right when clasping hands together.

65

Student Lesson 1

Human Genetic Variation

3. As students complete the inventory, direct their attention to the four

sets of labeled axes you prepared. Ask students to enter their data at

the appropriate place on each set of axes.

If you constructed the axes on the board, students can use chalk to

record their data. If you used masking tape to construct the axes on the

wall, ask students to record their data by taping one 3 × 5 card in the

appropriate place on each set of axes.

Tip from the field test: You may wish to give males one color of chalk or

3 × 5 card to use in recording their data and females a different color.

This strategy will allow the class to determine whether any of the three

characteristics other than sex (for example, height) shows differences

related to sex.

4. After the students have finished collecting and recording their data,

ask them to look at the four histograms they built and identify what

evidence they see in those data that they share many traits with other

members of their class.

Students may answer that all people have only one nose, and all people

are only one sex or the other.

5. Continue the lesson by saying, “But now that I look around the room,

it is clear that you are different. What evidence do you see in these

data that people are different?”

Students should recognize that not everyone is the same height and not

everyone has the same hair color.

As students look at the data, you may wish to ask them to compare the

shapes of the histograms for sex and height. The sex histogram has two

distinct peaks because there are only two categories of individuals—

female and male. That is, sex is a discontinuous trait. In contrast, height

is a continuous trait that has many categories of individuals, ranging

from very short to very tall. The shape of the height histogram may

begin to approach a bell curve, or normal distribution. It may also have

two peaks—a bimodal distribution—with one peak representing the

female students and the other representing the males.

6. Challenge students to try to describe just how different they are by

guessing how many traits they would have to consider to identify any

given student in the room as unique. Write the students’ predictions

on the board.

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7. Conduct the game described below with several volunteers.

• Choose a volunteer to determine his or her “uniqueness” as

compared with the other students.

• Ask all students to stand.

• Invite the volunteer to begin to identify his or her phenotype for

each of the 13 human traits listed on Master 1.1, An Inventory

of a Few Human Traits. Begin with the first trait and proceed

sequentially. As the volunteer lists his or her phenotype for each

trait, direct the students who share the volunteer’s phenotype

for that trait to remain standing. Direct all other students to sit.

• Continue in this fashion until the volunteer is the only person

still standing. Count how many traits the class had to consider

to distinguish the volunteer from all other students in the class.

Compare this number with the students’ predictions.

• Repeat as desired with another volunteer.

8. Ask students to work in pairs to answer the questions on Master 1.2,

Thinking about Human Variation.

Question 1. Some human traits can be changed by human intervention,

Collect and review the

and some cannot. Provide examples of each of these types of traits.

students’ completed

Biological sex and blood type cannot be changed. Hair color, skin

Master 1.2 to assess their

color, and even height and mental abilities can be changed by human

understanding of the

intervention. Students also may suggest that body piercing alters

lesson’s major concepts.

human traits.

Question 2. You probably already know that some traits are genetic and

others are environmental. But most human traits reflect an interaction

between genetic and environmental factors. Name some traits that might

fall into this category and explain why you think they do.

Height, weight, intelligence, and artistic or athletic ability are examples

Increasing evidence

of traits that are influenced by genetic and environmental factors. Some

indicates that all human

students may mention disorders such as certain types of cancer or

diseases have genetic and

even psychiatric disorders. We know that these types of traits are

environmental

both genetic and environmental because we see evidence that they

run in families and because we know we can modify them by

components. Point out

changing the environment.

that diseases such as

cancer, heart disease, and

Question 3. Describe some of the benefits of human genetic variation.

diabetes are traits that

What are some of the potential problems that such variation can cause?

show an interaction

between genetic and

Students may mention a number of benefits, such as allowing people

to be distinguished from one another and increasing the diversity of

environmental factors.

abilities, interests, and perspectives among humans. Some students may

Students will consider

recognize that genetic variation also benefits the species because it is

this concept in Lesson 4,

the basis for evolution by natural selection. Students will consider this

Are You Susceptible?

aspect of variation in Lesson 2, The Meaning of Genetic Variation.

67

Student Lesson 1

Human Genetic Variation

Expect students to recognize that just as being different from one

another has advantages, it also has disadvantages. For example, genetic

variation makes successful tissue and organ transplants more difficult

to accomplish than if we were all genetically identical. Students also

may note that the existence of real (or perceived) differences among

members of a population can allow prejudice and discrimination to

exist.

You may wish to point out that research reveals that more variation

exists within populations than between them (Figure 4, page 22). As

noted in “Understanding Human Genetic Variation” (pages 19–29), an

examination of human proteins demonstrated that about 85 percent

of all variation occurred within populations, whereas only 15 percent

occurred between populations. That is, we are more “like” people with

other ethnic or geographic origins than we might think.

9. Invite students to summarize the lesson’s major concepts by asking,

“What has this lesson illustrated about how one human compares

with another human? What has it illustrated about human variation

in general?”

These open-ended

Expect students to recognize that humans share many traits. Students

questions invite students

may also note that there is a wide range of variation in human traits and

to step back from the

one does not have to consider very many traits before a given person’s

lesson’s details to consider

uniqueness is demonstrated. Students should point out that some traits

its broader implications.

can be changed by human intervention and some cannot and that

Another way to invite

although some traits are genetic and others are environmental, most

such refl ection is to ask

human traits reflect an interaction between genetic and environmental

students to identify the

factors (that is, most are multifactorial). You may wish to introduce the

term “multifactorial” at this point; students will study multifactorial

most important or the

traits in more detail in Lesson 4, Are You Susceptible?

most interesting idea they

learned as a result of

Be sure that students generalize their responses to focus on variation

completing the lesson.

in populations, not variation simply between themselves and their

partners. Point out that the concept of variation in populations will

reappear in different, but less obvious, ways in the other lessons in

this module.

68

This lesson introduces students to several ideas that you may wish them to

Potential

explore in more depth. For example, assign students to use their textbooks

Extensions

to identify the biological mechanisms that lead to and maintain diversity

in populations.

Alternatively, ask students to list some of the advantages and disadvantages

of genetic variation in nonhuman populations. Invite them to locate and

report on cases where scientists are concerned that it may be diminishing

(for example, in domesticated crops and in populations of endangered

species being maintained in zoos and other protected settings).

Finally, to extend the discussion of the multifactorial nature of most human

traits, challenge students to suggest ways that scientists might investigate

the relative contributions that heredity and the environment make to such

traits (for example, twin studies or studies of adopted children in relation

to their adoptive and biological parents).

69

Student Lesson 1

Human Genetic Variation

Lesson 1 Organizer

Procedure

What the Teacher Does

Reference

Tell the class something like this: “If a visitor from another planet

Page 65

walked into this classroom, he might easily conclude that humans all Step 1

look very much alike.”

Ask, “Do you agree with this statement?” Use examples to help

students recognize that humans are more similar to each other than

they are to other species and that humans have a set of traits that

define us as a species.

Invite students to explore similarities among classmates. Give each

Page 65

student one copy of Master 1.1 and ask them to work in pairs to

Step 2

complete it.

Define terms (on page 65) if unfamiliar to students.

Ask students to enter data from their copy of Master 1.1 onto

Page 66

graphs you prepared on the board.

Step 3

Have class analyze data on graphs and identify evidence that

Page 66

supports the idea that students share many traits with other class

Step 4

members.

Continue by pointing out that you can see that individuals are

Page 66

different. Ask students, “What evidence supports that people are

Step 5

different?”

Ask students how many traits they would need to consider to

Page 66

identify a given student as unique. Write predictions on the board.

Step 6

Conduct the game described in Step 7 to determine uniqueness

Page 67

among class members.

Step 7

Give each student a copy of Master 1.2. Ask students to work in

Page 67

pairs to answer the questions. Discuss answers with the class (on

Step 8

pages 67 to 68).

Ask, “What has this lesson illustrated about how one human

Page 68

compares with another human? What has it illustrated about human Step 9

variation in general?” Ask students to summarize their ideas and

share with the class.

= Involves copying a master.

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index-79_1.png

index-79_2.png

L E S S O N 2

Explore

The Meaning of

Genetic Variation

Focus

At a Glance

Students investigate variation in the beta globin gene by identifying

base changes that do and do not alter function, and by using several

Web- or print-based resources to consider the significance in different

environments of the base change associated with sickle cell disease.

Major Concept

The ultimate source of genetic variation is differences in DNA sequences.

Most of those genetic differences do not affect how individuals function.

Some genetic variation, however, is associated with disease, and some

improves the ability of the species to survive changes in the environment.

Genetic variation, therefore, is the basis for evolution by natural selection.

Objectives

After completing this lesson, students will

• recognize that the extent of molecular variation between two people

is only about 0.1 percent, but because of the large size of the human

genome, this translates to about 3 million base differences;

• understand that most human genetic variation does not appear to

affect function;

• be able to explain that some human genetic variation is related to

disease and provide an example; and

• be able to describe a benefit of human genetic variation and relate

this benefit to human evolution by natural selection.

Prerequisite Knowledge

Students should understand basic Mendelian patterns of inheritance,

especially autosomal-recessive inheritance; the basic structure of DNA;

the transcription of DNA to messenger RNA; and the translation of

messenger RNA to protein.

Basic Science–Health Connection

Although the idea is made explicit only in annotations to teachers, this

lesson illustrates how advances in science and technology have allowed us

to establish relationships between some genetic variations and particular

phenotypes. For example, our understanding of the relationship between

DNA and protein has allowed us to establish a relationship between a

71

Human Genetic Variation

change in a single base pair and the symptomology of sickle cell disease.

Similarly, our understanding of the basic biochemical mechanisms

underlying the symptoms associated with sickle cell disease has provided

important clues about possible strategies for clinical intervention. You

may wish to make some of these points with your students as they

complete the lesson.

As discussed in Understanding Human Genetic Variation (pages 19–29),